National Seed Health System


ARCHIVED    Cf  7.1 – Gibberlla zeae

PATHOGEN: Gibberella zeae
HOST: Maize (Zea mays)
COMMON NAME: Gibberella ear and stalk rot
METHOD: Cf 7.1 Culture Plate (McGee, 1994)
SAMPLE: : 400 seeds


    1. Randomly select 400 seeds; 4 replicates of 100.
    2. Wash thoroughly in running water to remove chemical seed treatment.
    3. Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 1 minute; then triple rinse with sterile water.
    4. Aseptically place seeds on acid PDA (potato dextrose agar), usually 5 to 10 seeds per petri plate (9 cm diameter plates).
    5. Incubate samples at 25°C with 8 hours of light per day for 7 to 21 days. Check periodically to determine stage of colony growth.
    6. Suspect mycelium should be examined for the presence of perithecia or macroconidia of Gibberella zeae.


McGee, D. C. 1994. Seed assays for Stewart’s wilt and other seed-borne diseases of corn. Pages 161 – 168 in: Proc. Annu. Corn Sorghum Res. Conf. 48. American Seed Trade Association, Washington D. C.