ARCHIVED Cf 7.1 – Gibberlla zeae
|PATHOGEN: Gibberella zeae|
|HOST: Maize (Zea mays)|
|COMMON NAME: Gibberella ear and stalk rot|
|METHOD: Cf 7.1 Culture Plate (McGee, 1994)|
|METHOD CLASS: TEMPORARY STANDARD (B)|
|SAMPLE: : 400 seeds|
- Randomly select 400 seeds; 4 replicates of 100.
- Wash thoroughly in running water to remove chemical seed treatment.
- Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 1 minute; then triple rinse with sterile water.
- Aseptically place seeds on acid PDA (potato dextrose agar), usually 5 to 10 seeds per petri plate (9 cm diameter plates).
- Incubate samples at 25°C with 8 hours of light per day for 7 to 21 days. Check periodically to determine stage of colony growth.
- Suspect mycelium should be examined for the presence of perithecia or macroconidia of Gibberella zeae.
McGee, D. C. 1994. Seed assays for Stewart’s wilt and other seed-borne diseases of corn. Pages 161 – 168 in: Proc. Annu. Corn Sorghum Res. Conf. 48. American Seed Trade Association, Washington D. C.