NSHS Methods List & Codes

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DATE: Current as of 09.10.2020

Crop Pathogen Method Code Former Code Class NSHS Version Date
Bean Pseudomonas syringae pv. phaseolicola Dilution and plating, followed by PCR (ISTA 7-023: ver 1.3) Be 1.2   St 1.3 1/1/2020
  Pseudomonas syringae pv. syringae Agar Plate Assay (Mohan and Schaad, 1987) Be 2.1   TSt 1.0 12/2012
  Xanthomonas axonopodis pv. phaseoli ISTA Method (7-021: ver 3.1) Be 3.1   TSt 1.3 1/1/2020
  Curtobacterium flaccumfaciens pv. flaccumfaciens PCR Be 4.1   TSt 1.0 12/2012
  Curtobacterium flaccumfaciens pv. flaccumfaciens Selective Media Assay (Maringoni et al., 2006) Be 4.2   TSt 1.0 12/2012
  Curtobacterium flaccumfaciens pv. flaccumfaciens Selective Media Assay (Univ. of Idaho) Be 4.3   TSt 1.0 12/2012
  Colletotrichum lindemuthianum ISTA Method (7-006: ver 1.3) Be 5.1   TSt 1.1 1/1/2020
 Pea Ascochyta pisi ISTA Method (7-005: ver 1.2) Pe 1.1   St 1.1 1/1/2020
  Ascochyta pinodes Semi-selective Agar Pe 1.2   TSt 1.0 05/2013
  Ascochyta pinodes Blotter/Agar Pe 1.3   TSt 1.0 05/2013
  Ascochyta pinodella ISTA Method (7-005: ver 1.2) Pe 1.4   TSt 1.1 1/1/2020
  Pea Seedborne Mosaic Virus ISTA Method (7-024: ver 1.3) Pe 2.1   St 1.1 1/1/2020
  Pea Early Browning Virus ISTA Method (7-024: ver 1.3) Pe 3.1   St 1.1 1/1/2020
  Pseudomonas syringae pv pisi ISTA Method (7-029: ver 1.1) Pe 4.1   St 1.1 1/1/2020
Brassica Xanthomonas campestris pv. campestris ISTA Method (7-019A: ver 6.0; 7-019B: ver 1.2) Br 1.1   St 1.2 1/1/2019
  Phoma lingam Freeze Blotter (ISTA 7-004: ver 2.0) Br 2.1   St 1.2 1/1/2020
 Carrots Xanthomonas campestris pv. carotae ISTA Method (7-020: ver 1.3) Rb 1.1   St 1.0 1/1/2020
  Alternaria dauci ISTA Method (7-001A: ver 1.4; 7-001B: ver 1.4) Rb 2.1   St 1.1 1/1/2020
  Alternaria dauci Direct Plating/Blotter Method (Sunseeds) Rb 2.2   St 1.0 2001
  Alternaria radicina ISTA Method (7-002A: ver 1.3) Rb 3.1   St 1.1 1/1/2020
 Cucurbits Acidovorax avenae ssp. citrulli Seedling grow-out Cb 1.1   St 1.1 2001
  Acidovorax avenae ssp. citrulli CSPL PCR Assay Cb 1.5   TSt (oc) 1.0 12/2012
  Acidovorax citrulli ISHI Method Cb 1.6   St 1.0 07/2017
  Didymella bryoniae PCR Cb 2.1   TSt 1.0 12/2012
  Didymella bryoniae Vegetables Blotter Assay Cb 2.2   TSt 1.0 2001
  Cucumber green mottle mosaic virus ISTA Method (7-026: ver 1.3) Cb 3.1   TSt 1.1 1/1/2020
  Melon necrotic spot virus ISTA Method (7-026: ver 1.3) Cb 4.1   TSt 1.1 1/1/2020
  Squash mosaic virus (SMV) ISTA Method (7-026: ver 1.3) Cb 5.1   TSt 1.1 1/1/2020
  Fusarium oxysporum f.sp. niveum Freeze Blotter (Syngenta) Cb 6.1   TSt 1.0 11/2014
 Celery Septoria apiicola Seed Wash (STA Laboratories) Lcb 1.1   St 1.0 2001
  Phoma apiicola Grow-out (Hewitt, 1971) Lcb 2.1   TSt 1.0 2001
 Spinach Peronospora farinosa f.sp. spinaciae Wash Test (Inaba et al., 1983) Lcb 3.1   TSt 1.0 12/2012
  Peronospora farinosa f.sp. spinaciae Wash Test (CDFA) Lcb 3.2   St 1.0 12/2012
  Verticillium dahliae Freeze Blotter, ISTA Method 7-032: ver 1.0  Lcb 4.1   St 1.1 1/1/2020
  Verticillium dahliae NP-10 Agar, ISTA Method 7-032: ver 1.0  Lcb 4.2   St 1.1 1/1/2020
  Cucumber mosaic virus Seedling grow-out and ELISA Lcb 5.1   St 1.0 12/2012
  Stemphylium botryosum f. sp. spinacia Freeze Blotter Lcb 6.1   St 1.0 12/2012
 Coriander Pseudomonas syringae pv. coriandricola Selective Media Test (STA Laboratories) Lcb 7.1   St 1.0 2001
 Lettuce Lettuce mosaic virus Chenopodium (Kimble et al., 1975) Lcb 8.1  Cv 1.1 St 1.0 2001
  Lettuce mosaic virus ISHI-Vegetables Method (version 4.3, Feb 2017) Lcb 8.2  Cv 1.2 St 1.1 5/11/2017
 Corn Salad Acidovorax valerianellae ISTA Method ( 7-030: ver 1.1) Lcb 9.1   St 1.0 1/1/2020
 Solanaceae (Tomato/Pepper) Xanthomonas spp. ISHI-Vegetables Method (pepper, version 5, 2013) So 1.1   TSt 1.1 05/2020
  Xanthomonas spp. ISHI-Vegetables Method (tomato, version 4, 2011) So 1.2   TSt 1.1 05/2020
  Clavibacter michiginensis ssp. michiginensis ISHI-Vegetables Method (version 4.3, 2015) So 2.1   St 1.0 12/2015
  Pseudomonas syringae pv. tomato CDFA Method So 3.1   St 1.0 2001
  Pepino Mosaic Virus ISHI Method (version 4, 2011) So 4.1   TSt 1.0 12/2013
  Tobamoviruses (TMV, ToMV, PMMov) ISTA Method (7-028: ver 1.2) So 5.1   TSt 1.2 1/1/2020
  Pospiviroids (CLVd, PCFVd, PSTVd, TASVd, TCDVd and TPMVd) TaqMan RT-PCR Method, National Seed Health System (NSHS) So 6.1   St 1.0 6/2020
 Onion Alternaria porri Agar Plate Method On 1.1   TSt 1.0 12/2013
  Stemphylium vesicarium S. vesicarium On 2.1   TSt 1.0 12/2013
 Maize Stenocarpella maydis ISU Culture plate Mz 1.1  Cf 1.1 St 1.0 2001
  Cochliobolus carbonum ISU Freezing Blotter Method (McGee, 1994) Mz 2.1  Cf 2.1 St 1.0 2001
  Cochliobolus heterostrophus ISU Freezing Blotter Method (McGee, 1994) Mz 3.1  Cf 3.1 St 1.0 2001
  Ustilago maydis/Sphacelotheca reiliana Wash Test (McGee, 1988) Mz 4.1  Cf 4.1 St 1.0 2001
  Sclerophthora macrospora Direct Visual method (CIMMYT) Mz 5.1  Cf 5.1 TSt 1.0 2001
  Peronosclerospora sorghi Grow-out (Adenle and Cardwell, 2000) Mz 6.1  Cf 6.1 St 1.0 2001
  Fusarium Blotter (Singh et al., 1974) Mz 7.1  Cf 8.2 St 1.0 2001
  Penicillium oxalicum Blotter test (Handoo and Aulakh, 1979) sCNS Mz 8.1  Cf 9.1 St 1.0 2001
  Clavibacter michiganensis ssp. nebraskensis Culture Plate Method (Shepherd, 1999) Mz 9.1  Cb 1.1 St 1.0 2001
  Erwinia stewartii ELISA (Lamka et al., 1991) Mz 10.1  Cb 2.1 St 1.1 9/21/2016
  Maize Dwarf Mosaic Virus ELISA (Iowa State University/Agdia Inc kit) Mz 11.1  Cb 3.1 TSt 1.1 9/21/2016
  Maize Chlorotic Mottle Virus ELISA (Iowa State University/Agdia Inc kit) Mz 12.1  Cb 4.1 TSt 1.1 9/21/2016
  Cercospora zeae-maydis Culture Plate Mz 13.1  Cf 10.1 RNSP 1.0 2001
  Kabateilla zeae Culture Plate Mz 14.1  Cf 11.1 RNSP 1.0 2001
  Phyllosticta maydis Culture Plate Mz 15.1  Cf 12.1 RNSP 1.0 2001
 Soybean Cercospora kikuchii Culture Plate (Jordan et al., 1986) Sb 1.1  Sf 1.1 St 1.0 2001
  Cercospora kikuchii Culture Plate (McGee and Nyvall, 1984) Sb 1.2  Sf 1.2 St 1.0 2001
  Phomopsis/Diaporthe spp. Culture plate (McGee, 1986) Sb 2.1  Sf 2.1 St 1.0 2001
  Phomopsis/Diaporthe spp. Blotter (McGee and Nyvall, 1984) Sb 2.2  Sf 2.2 St 1.0 2001
  Sclerotinia sclerotiorum Culture plate (Totir, 2000) Sb 3.1  Sf 3.1 St 1.0 2001
  Pseudomonas syringae pv. glycinea Soaked bulk seed – Biochemical confirmation Sb 4.1  Sb 2.1 St 1.0 2001
  Pseudomonas syringae pv. glycinea Ground bulk seed – Serological and pathogenicity Sb 4.2  Sb 2.2 St 1.0 2001
  Tobacco Ringspot Virus ELISA (Iowa State University/Agdia Inc kit) Sb 5.1  Sv 1.1 TSt 1.1 9/22/2016
  Tomato Ringspot Virus ELISA (Iowa State University/Agdia Inc kit) Sb 6.1  Sv 2.1 TSt 1.1 9/22/2016
  Soybean Mosaic Virus ELISA (Iowa State University/Agdia Inc kit) Sb 7.1  Sv 3.1 TSt 1.1 9/22/2016
  Bean Pod Mottle Virus ELISA (Iowa State University/Agdia Inc kit) Sb 8.1  Sv 4.1 TSt 1.1 9/22/2016
  Phakopsora pachyrhizi Wash test Sb 9.1  Sf 4.1 RNSP 1.0 2001
  Phialophora gregatum Culture Plate Sb 10.1  Sf 5.1 RNSP 1.0 2001
  Phytophthora megasperma var. glycinea Culture Plate Sb 11.1  Sf 6.1 RNSP 1.0 2001

Approved methods are classified as follows:

St: Standard (A)
TSt: Temporary standard (B)
RNSP: Regulated non-seedborne pathogens