| Bean |
Pseudomonas syringae pv. phaseolicola |
Dilution and plating, followed by Pathogenicity Test (ISTA 7-023: ver 1.5) |
Be 1.2 |
|
St |
1.5 |
3/1/2024 |
|
Pseudomonas syringae pv. syringae |
Agar Plate Assay (Mohan and Schaad, 1987) |
Be 2.1 |
|
TSt |
1.0 |
12/2012 |
|
Xanthomonas axonopodis pv. phaseoli |
ISTA Method (7-021: ver 3.3) |
Be 3.1 |
|
St |
1.5 |
3/1/2024 |
|
Curtobacterium flaccumfaciens pv. flaccumfaciens |
Selective Media Assay |
Be 4.3 |
|
TSt |
1.2 |
4/2025 |
|
Colletotrichum lindemuthianum |
ISTA Method (7-006: ver 1.6) |
Be 5.1 |
|
TSt |
1.3 |
3/1/2024 |
| Pea |
Ascochyta pisi |
ISTA Method (7-005: ver 1.5) |
Pe 1.1 |
|
St |
1.3 |
3/1/2024 |
|
Pea Seedborne Mosaic Virus |
ISTA Method (7-024: ver 1.5) |
Pe 2.1 |
|
St |
1.3 |
3/1/2024 |
|
Pea Early Browning Virus |
ISTA Method (7-024: ver 1.5) |
Pe 3.1 |
|
St |
1.3 |
3/1/2024 |
|
Pseudomonas syringae pv pisi |
ISTA Method (7-029: ver 1.3) |
Pe 4.1 |
|
St |
1.3 |
3/1/2024 |
| Brassica |
Xanthomonas campestris pv. campestris |
ISTA Method (7-019a: ver 6.0; 7-019b: ver 1.3) |
Br 1.1 |
|
St |
1.3 |
1/1/2022 |
|
Phoma lingam |
Freeze Blotter (ISTA 7-004: ver 2.2) |
Br 2.1 |
|
St |
1.4 |
3/1/2024 |
| Carrots |
Xanthomonas hortorum pv. carotae |
NSHS Method |
Rb 1.1 |
|
St |
2.1 |
2/19/2024 |
|
Alternaria dauci |
ISTA Method (7-001a: ver 1.6; 7-001b: ver 1.6) |
Rb 2.1 |
|
St |
1.3 |
3/1/2024 |
|
Alternaria dauci |
Direct Plating/Blotter Method (Sunseeds) |
Rb 2.2 |
|
St |
1.0 |
2002 |
|
Alternaria radicina |
ISTA Method (7-002a: ver 1.5) |
Rb 3.1 |
|
St |
1.3 |
3/1/2024 |
| Cucurbits |
Acidovorax citrulli |
Seedling grow-out |
Cb 1.1 |
|
St |
1.1 |
2017 |
|
Acidovorax citrulli |
CSPL PCR Assay |
Cb 1.5 |
|
TSt |
1.0 |
12/2012 |
|
Acidovorax citrulli |
ISHI Method (version 2.0, May 2021) |
Cb 1.6 |
|
St |
2.0 |
05/2021 |
|
Didymella bryoniae |
PCR (Ling et al., 2010) |
Cb 2.1 |
|
TSt |
1.0 |
12/2012 |
|
Didymella bryoniae |
Vegetables Blotter Assay |
Cb 2.2 |
|
TSt |
1.0 |
2001 |
|
Cucumber green mottle mosaic virus |
ISTA Method (7-026: ver 1.5) |
Cb 3.1 |
|
TSt |
1.3 |
3/1/2024 |
|
Melon necrotic spot virus |
ISTA Method (7-026: ver 1.5) |
Cb 4.1 |
|
TSt |
1.3 |
3/1/2024 |
|
Squash mosaic virus (SMV) |
ISTA Method (7-026: ver 1.5) |
Cb 5.1 |
|
TSt |
1.3 |
3/1/2024 |
|
Fusarium oxysporum f.sp. niveum |
Freeze Blotter (Syngenta) |
Cb 6.1 |
|
TSt |
1.0 |
11/2014 |
| Celery |
Septoria apiicola |
Seed Wash (STA Laboratories) |
Lcb 1.1 |
|
St |
1.0 |
2001 |
|
Phoma apiicola |
Grow-out (Hewitt, 1971) |
Lcb 2.1 |
|
TSt |
1.0 |
2001 |
| Spinach |
Peronospora farinosa f.sp. spinaciae |
Wash Test (Inaba et al., 1983) |
Lcb 3.1 |
|
TSt |
1.0 |
12/2012 |
|
Peronospora farinosa f.sp. spinaciae |
Wash Test (CDFA) |
Lcb 3.2 |
|
TSt |
1.0 |
12/2012 |
|
Verticillium dahliae |
Freeze Blotter, ISTA Method (7-032: ver 1.3) |
Lcb 4.1 |
|
St |
1.3 |
3/1/2024 |
|
Verticillium dahliae |
NP-10 Agar, ISTA Method (7-032: ver 1.3) |
Lcb 4.2 |
|
St |
1.3 |
3/1/2024 |
|
Cucumber mosaic virus |
Seedling grow-out and ELISA (Yang et al., 1997) |
Lcb 5.1 |
|
St |
1.0 |
12/2012 |
|
Stemphylium botryosum f. sp. spinacia |
Freeze Blotter (Hernandez-Perez and du Toit, 2016) |
Lcb 6.1 |
|
St |
1.0 |
12/2012 |
| Coriander |
Pseudomonas syringae pv. coriandricola |
Selective Media Test (STA Laboratories) |
Lcb 7.1 |
|
St |
1.0 |
2001 |
| Lettuce |
Lettuce mosaic virus |
Chenopodium Assay (Kimble et al., 1975) |
Lcb 8.1 |
Cv 1.1 |
St |
1.0 |
2001 |
|
Lettuce mosaic virus |
ISHI-Vegetables Method (version 4.3, July 2017) |
Lcb 8.2 |
Cv 1.2 |
St |
1.1 |
6/04/2021 |
| Corn Salad |
Acidovorax valerianellae |
ISTA Method ( 7-030: ver 1.3) |
Lcb 9.1 |
|
St |
1.2 |
3/1/2024 |
| Solanaceae (Tomato/Pepper) |
Xanthomonas spp. |
ISHI-Vegetables Method (pepper, version 5, 2013) |
So 1.1 |
|
TSt |
1.2 |
05/2022 |
|
Xanthomonas spp. |
ISHI-Vegetables Method (tomato, version 4, 2011) |
So 1.2 |
|
TSt |
1.2 |
05/2022 |
|
Clavibacter michiganensis ssp. michiganensis |
ISHI-Vegetables Method (version 4.3.1, 2017) |
So 2.1 |
|
St |
1.1 |
07/2021 |
|
Pseudomonas syringae pv. tomato |
CDFA Method |
So 3.1 |
|
St |
1.0 |
2001 |
|
Pepino Mosaic Virus |
ISHI Method (version 5 2021) |
So 4.1 |
|
TSt |
2.0 |
05/2021 |
|
Tobamoviruses (TMV, ToMV, PMMov) |
ISTA Method (7-028: ver 1.4)-Tomato; ISF Jan 2007-Pepper |
So 5.1 |
|
TSt |
1.4 |
3/1/2024 |
|
Pospiviroids (CLVd, PCFVd, PSTVd, TASVd, TCDVd and TPMVd) |
TaqMan RT-PCR Method, National Seed Health System (NSHS) |
So 6.1 |
|
St |
1.4 |
03/2024 |
|
Tomato Brown Rugose (ToBRFV) |
TaqMan RT-PCR Method, National Seed Health System (NSHS) |
So 7.1 |
|
St |
2.2 |
02/2024 |
| Onion |
Alternaria porri |
Agar Plate Method (Aveling et al., 1993) |
On 1.1 |
|
TSt |
1.0 |
12/2013 |
|
Stemphylium vesicarium |
S. vesicarium |
On 2.1 |
|
TSt |
1.0 |
12/2013 |
| Maize |
Stenocarpella maydis |
Culture plate (ISU) |
Mz 1.1 |
Cf 1.1 |
St |
1.0 |
2001 |
|
Cochliobolus carbonum |
ISU Freezing Blotter Method (McGee, 1994) |
Mz 2.1 |
Cf 2.1 |
St |
1.0 |
2001 |
|
Cochliobolus heterostrophus |
ISU Freezing Blotter Method (McGee, 1994) |
Mz 3.1 |
Cf 3.1 |
St |
1.0 |
2001 |
|
Ustilago maydis/Sphacelotheca reiliana |
Wash Test (McGee, 1988) |
Mz 4.1 |
Cf 4.1 |
St |
1.0 |
2001 |
|
Sclerophthora macrospora |
Direct Visual method (CIMMYT) |
Mz 5.1 |
Cf 5.1 |
St |
1.0 |
2001 |
|
Peronosclerospora sorghi |
Grow-out (Adenle and Cardwell, 2000) |
Mz 6.1 |
Cf 6.1 |
St |
1.0 |
2001 |
|
Fusarium |
Blotter (Singh et al., 1974) |
Mz 7.1 |
Cf 8.2 |
St |
1.0 |
2001 |
|
Penicillium oxalicum |
Blotter test (Handoo and Aulakh, 1979) |
Mz 8.1 |
Cf 9.1 |
St |
1.0 |
2001 |
|
Clavibacter michiganensis ssp. nebraskensis |
sCNS Culture Plate Method (Shepherd, 1999) |
Mz 9.1 |
Cb 1.1 |
St |
1.0 |
2001 |
|
Pantoea stewartii |
ELISA (Lamka et al., 1991) |
Mz 10.1 |
Cb 2.1 |
St |
2.2 |
03/2025 |
|
Maize Dwarf Mosaic Virus |
ELISA (Iowa State University/Agdia Inc kit) |
Mz 11.1 |
Cb 3.1 |
TSt |
1.2 |
10/2020 |
|
Maize Chlorotic Mottle Virus |
ELISA |
Mz 12.1 |
Cb 4.1 |
TSt |
1.2 |
10/2020 |
|
Cercospora zeae-maydis |
Culture Plate |
Mz 13.1 |
Cf 10.1 |
RNSP |
1.0 |
2001 |
|
Kabatiella zeae |
Culture Plate |
Mz 14.1 |
Cf 11.1 |
RNSP |
1.0 |
2001 |
|
Phyllosticta maydis |
Culture Plate |
Mz 15.1 |
Cf 12.1 |
RNSP |
1.0 |
2001 |
| Soybean |
Cercospora kikuchii |
Culture Plate (Jordan et al., 1986) |
Sb 1.1 |
Sf 1.1 |
St |
1.0 |
2001 |
|
Cercospora kikuchii |
Blotter (McGee and Nyvall, 1984) |
Sb 1.2 |
Sf 1.2 |
St |
1.0 |
2001 |
|
Phomopsis/Diaporthe spp. |
Culture plate (McGee, 1986) |
Sb 2.1 |
Sf 2.1 |
St |
1.0 |
2001 |
|
Phomopsis/Diaporthe spp. |
Blotter (McGee and Nyvall, 1984) |
Sb 2.2 |
Sf 2.2 |
St |
1.0 |
2001 |
|
Sclerotinia sclerotiorum |
Culture plate (Totir, 2000) |
Sb 3.1 |
Sf 3.1 |
St |
1.0 |
2001 |
|
Pseudomonas syringae pv. glycinea |
Soaked bulk seed – Biochemical confirmation |
Sb 4.1 |
Sb 2.1 |
St |
1.1 |
2022 |
|
Pseudomonas syringae pv. glycinea |
Ground bulk seed – Serological and pathogenicity |
Sb 4.2 |
Sb 2.2 |
St |
1.1 |
2022 |
|
Tobacco Ringspot Virus |
ELISA (Iowa State University/Agdia Inc kit) |
Sb 5.1 |
Sv 1.1 |
TSt |
1.2 |
10/2020 |
|
Tomato Ringspot Virus |
ELISA (Iowa State University/Agdia Inc kit) |
Sb 6.1 |
Sv 2.1 |
TSt |
1.2 |
10/2020 |
|
Soybean Mosaic Virus |
ELISA (Iowa State University/Agdia Inc kit) |
Sb 7.1 |
Sv 3.1 |
TSt |
1.2 |
10/2020 |
|
Bean Pod Mottle Virus |
ELISA (Iowa State University/Agdia Inc kit) |
Sb 8.1 |
Sv 4.1 |
TSt |
1.2 |
10/2020 |
