SEED HEALTH TESTING METHODS2020-05-14T12:10:55-05:00

NSHS ACCREDITED

Seed Health Testing Methods

The National Seed Health System (NSHS) laboratory-based Seed Health Testing methods examine for plant pathogens in seeds. A comprehensive list of approved NSHS Seed Health Testing Methods & Codes are published here:

NSHS METHODS & CODES

BEANS

Be 1.2   Pseudomonas syringae pv phaseolicola
Be 2.1   Pseudomonas syringae pv syringae
Be 3.1   Xanthomonas axonopodis pv phaseoli
Be 4.1   Curtobacterium flaccumfaciens pv flaccumfaciens
Be 4.2   Curtobacterium flaccumfaciens pv flaccumfaciens
Be 4.3   Curtobacterium flaccumfaciens pv flaccumfaciens
Be 5.1   Colletotrichum lindemuthianum

PEAS

Pe 1.1   Ascochyta pisi
Pe 1.2   Ascochyta pinodes – agar plate
Pe 1.3   Ascochyta pinodes – blotter/agar
Pe 1.4   Ascochyta pinodella
Pe 2.1   Pea Seedborne Mosaic Virus
Pe 3.1   Pea Early Browning Virus
Pe 4.1   Pseudomonas syringae pv pisi

BRASSICA

Br 1.1  Xanthomonas campestris pv campestris
Br 2.1  Phoma lingam

CARROT

Rb 1.1   Xanthomonas campestris pv carotae
Rb 2.1   Alternaria dauci – ISTA 7-001A
Rb 2.2   Alternaria dauci – blotter
Rb 3.1   Alternaria radicina

CUCURBIT

Cb 1.1   Acidovorax avenae ssp citrulli – Grow-out
Cb 1.5   Acidovorax avenae ssp citrulli – Seedling PCR
Cb 1.6   Acidovorax citrulli – ISHI Method
Cb 2.1    Didymella bryoniae – PCR
Cb 2.2    Didymella bryoniae – blotter
Cb 3.1    Cucumber Green Mottle Mosaic Virus
Cb 4.1    Melon Necrotic Spot Virus
Cb 5.1    Squash Mosaic Virus
Cb 6.1    Fusarium oxysporum f sp niveum

CELERY

Lcb 1.1    Septoria apiicola
Lcb 2.1    Phoma apiicola

SPINACH

Lcb 3.1    Peronospora farinosa f sp spinaciae – wash test
 Lcb 3.2    Peronospora farinosa f sp spinaciae – wash test
Lcb 4.1    Verticillium dahliae – Freeze blotter
Lcb 4.2    Verticillium dahliae – NP-10 agar
Lcb 5.1    Cucumber Mosaic Virus
Lcb 6.1    Stemphylium botryosum fsp spinacia

CORIANDER

Lcb 7.1    Pseudomonas syringae pv coriandricola

LETTUCE

Lcb 8.1    Lettuce Mosaic Virus – Bioassay on chenopodium
Lcb 8.2    Lettuce Mosaic Virus – ISHI Method

CORN SALAD

Lcb 9.1    Acidovorax valerianellae – ISTA Assay 7-030

TOMATO/PEPPER

So 1.1    Xanthomonas spp. – pepper
So 1.2    Xanthomonas spp. – tomato
So 2.1    Clavibacter michiganensis ssp michiganensis – tomato
So 3.1    Pseudomonas syringae pv tomato – tomato
So 4.1    Pepino Mosaic Virus – tomato​
So 5.1    Tobamovirus – tomato-pepper

ONION

On 1.1    Alternaria porri
On 2.1    Stemphylium vesicarium

MAIZE

Mz 1.1     Stenocarpella maydis
Mz 2.1     Cochliobolus carbonum
Mz 3.1     Cochliobolus heterostrophus
Mz 4.1     U. maydis and S. reiliana
Mz 5.1     Sclerophthora macrospora
Mz 6.1     Peronosclerospora sorghi
Mz 7.1     Fusarium
Mz 8.1     Penicillium oxalicum
Mz 9.1     Clavibacter michiganensis ssp nebraskensis
Mz 10.1   Pantoea stewartii
Mz 11.1   Maize Dwarf Mosaic Virus
Mz 12.1   Maize Chlorotic Mottle Virus
Mz 13.1   Cercospora zeae-maydis
Mz 14.1   Kabatiella zeae
Mz 15.1   Phyllosticta maydis

SOYBEAN

Sb 1.1       Cercospora kikuchii – Agar plate
Sb 1.2       Cercospora kikuchii – Blotter
Sb 2.1       Phomopsis-Diaporthe – Agar plate
Sb 2.2       Phomopsis-Diaporthe – Blotter
Sb 3.1       Sclerotinia sclerotiorum
Sb 4.1       Pseudomonas syringae pv glycinea – Soaked bulk seed
Sb 4.2       Pseudomonas syringae pv glycinea – Ground bulk seed
Sb 5.1       Tobacco Ringspot Virus
Sb 6.1       Tomato Ringspot Virus
Sb 7.1       Soybean Mosaic Virus
Sb 8.1       Bean Pod Mottle Virus
Sb 9.1       Phakopsora pachyrhizi
Sb 10.1     Phialophora gregatum
Sb 11.1     Phytophthora megasperma fp. glycinea

ARCHIVED METHODS

“Archived methods” are methods that were classified as Temporary Standard methods (B methods) but were not listed on the accreditation of any NSHS participants. These methods have been removed from the official NSHS list of methods and are not to be used for phytosanitary certification unless separate APHIS authorization is given. 

ARCHIVED METHODS