NSHS ACCREDITED
Seed Health Testing Methods
The National Seed Health System (NSHS) laboratory-based Seed Health Testing methods examine for plant pathogens in seeds. A comprehensive list of approved NSHS Seed Health Testing Methods & Codes are published here:
Be 1.2 Pseudomonas syringae pv phaseolicola
Be 2.1 Pseudomonas syringae pv syringae
Be 3.1 Xanthomonas axonopodis pv phaseoli
Be 4.1 Curtobacterium flaccumfaciens pv flaccumfaciens
Be 4.2 Curtobacterium flaccumfaciens pv flaccumfaciens
Be 4.3 Curtobacterium flaccumfaciens pv flaccumfaciens
Be 5.1 Colletotrichum lindemuthianum
Cb 1.1 Acidovorax avenae ssp citrulli – Grow-out
Cb 1.5 Acidovorax avenae ssp citrulli – Seedling PCR
Cb 1.6 Acidovorax citrulli – ISHI Method
Cb 2.1 Didymella bryoniae – PCR
Cb 2.2 Didymella bryoniae – blotter
Cb 3.1 Cucumber Green Mottle Mosaic Virus
Cb 4.1 Melon Necrotic Spot Virus
Cb 5.1 Squash Mosaic Virus
Cb 6.1 Fusarium oxysporum f sp niveum
Mz 2.1 Cochliobolus carbonum
Mz 3.1 Cochliobolus heterostrophus
Mz 4.1 U. maydis and S. reiliana
Mz 5.1 Sclerophthora macrospora
Mz 6.1 Peronosclerospora sorghi
Mz 7.1 Fusarium
Mz 8.1 Penicillium oxalicum
Mz 9.1 Clavibacter michiganensis ssp nebraskensis
Mz 10.1 Pantoea stewartii
Mz 11.1 Maize Dwarf Mosaic Virus
Mz 12.1 Maize Chlorotic Mottle Virus
Mz 13.1 Cercospora zeae-maydis
Mz 14.1 Kabatiella zeae
Mz 15.1 Phyllosticta maydis
Sb 1.2 Cercospora kikuchii – Blotter
Sb 2.1 Phomopsis-Diaporthe – Agar plate
Sb 2.2 Phomopsis-Diaporthe – Blotter
Sb 3.1 Sclerotinia sclerotiorum
Sb 4.1 Pseudomonas syringae pv glycinea – Soaked bulk seed
Sb 4.2 Pseudomonas syringae pv glycinea – Ground bulk seed
Sb 5.1 Tobacco Ringspot Virus
Sb 6.1 Tomato Ringspot Virus
Sb 7.1 Soybean Mosaic Virus
Sb 8.1 Bean Pod Mottle Virus
Sb 9.1 Phakopsora pachyrhizi
Sb 10.1 Phialophora gregatum
Sb 11.1 Phytophthora megasperma fp. glycinea
“Archived methods” are methods that were classified as Temporary Standard methods (B methods) but were not listed on the accreditation of any NSHS participants. These methods have been removed from the official NSHS list of methods and are not to be used for phytosanitary certification unless separate APHIS authorization is given.