Archived 04.03.2018 Be 2.2 – Pseudomonas syringae pv syringae
| Version | 1.0 |
|---|---|
| Date | 12/2012 [Archived 04.03.2018] |
| Pathogen | Pseudomonas syringae pv. syringae |
| Host | common bean (Phaseolus vulgaris) |
| Common Name | bacterial brown spot |
| Method | Be 2.2 Modified Agar Plate Assay (Hunter and Ludwig, 1991) |
| Method Class | Temporary Standard (B) |
| Sample | 1 kg of seeds |
Procedure:
- One kilogram of seed is soaked in 3L of sterile saline solution with 0.01% Tween 20 for 20 hours at 20°C while being aerated.
- Ten-fold dilutions are made from the soaking solution and plated onto selective media KBC and MSP in triplicate.
- Plates are incubated at room temperature.
- Presumptive positive colonies are confirmed by biochemical and pathogenicity testing.
References:
Hunter and Ludwig, 1991. A modified procedure for assaying bean seeds for the pathogen causing bacterial brown spot and results of assays of commercial bean seeds. Annual Report Bean Improvement Cooperative. 34:21-22.