National Seed Health System
ARCHIVED 04.03.2018 Be 2.2 – Pseudomonas syringae pv syringae
| VERSION: 1.0 |
| DATE: 12/2012 [ARCHIVED 04.03.2018] |
| PATHOGEN: Pseudomonas syringae pv. syringae |
| HOST: common bean (Phaseolus vulgaris) |
| COMMON NAME: bacterial brown spot |
| METHOD: Be 2.2 Modified Agar Plate Assay (Hunter and Ludwig, 1991) |
| METHOD CLASS: TEMPORARY STANDARD (B) |
| SAMPLE: 1 kg of seeds |
PROCEDURE:
1. One kilogram of seed is soaked in 3L of sterile saline solution with 0.01% Tween 20 for 20 hours at 20°C while being aerated.
2. Ten-fold dilutions are made from the soaking solution and plated onto selective media KBC and MSP in triplicate.
3. Plates are incubated at room temperature.
4. Presumptive positive colonies are confirmed by biochemical and pathogenicity testing.
REFERENCES:
Hunter and Ludwig, 1991. A modified procedure for assaying bean seeds for the pathogen causing bacterial brown spot and results of assays of commercial bean seeds. Annual Report Bean Improvement Cooperative. 34:21-22.