Sb 1.1 Cercospora kikuchii – Agar plate 2017-05-19T16:18:30+00:00

National Seed Health System

[printfriendly]

 Sb 1.1  Cercospora kikuchii – Agar plate

VERSION: 1.0
DATE: 2001
PATHOGEN: Cercospora kikuchii
HOST: purple seed stain
COMMON NAME: halo blight
METHOD: Sb 1.1 Culture Plate (Jordan et al., 1986) (formerly Sf 1.1)
METHOD CLASS: STANDARD (A)
SAMPLE: 400 seeds

PROCEDURE:

1. Surface sterilize seeds in 0.5% sodium hypochlorite (NaOCl) for 4 minutes; wash in distilled water.

2. Plate on potato dextrose agar.

3. Incubate at 25°C in the dark for 10 days.

REFERENCES:

Jordan, E. G., Manandhar, J. B., Thapliyal, P.N. and Sinclair, J. B. 1986. Factors affecting soybean seed quality in Illinois. Plant Disease. 70(3):246-248.