Seed Health Testing Methods

Section

Methods

The National Seed Health System (NSHS) laboratory-based Seed Health Testing methods examine for plant pathogens in seeds. A comprehensive list of approved NSHS Seed Health Testing Methods & Codes are published here:

NSHS Methods and Codes

Beans

Be 1.2 Pseudomonas syringae pv phaseolicola (PDF)

Be 2.1 Pseudomonas syringae pv syringae (PDF)

Be 3.1 Xanthomonas axonopodis pv phaseoli (PDF)

Be 4.3 Curtobacterium flaccumfaciens pv flaccumfaciens (PDF)

Be 5.1 Colletotrichum lindemuthianum (PDF)

Peas

Pe 1.1 Ascochyta pisi (PDF)

Pe 2.1 Pea Seedborne Mosaic Virus (PDF)

Pe 3.1 Pea Early Browning Virus (PDF)

Pe 4.1 Pseudomonas syringae pv pisi (PDF)

Brassica

Br 1.1 Xanthomonas campestris pv campestris (PDF)

Br 2.1 Phoma lingam (PDF)

Carrot

Rb 1.1 Xanthomonas campestris pv carotae (PDF)

Rb 2.1 Alternaria dauci (PDF)

Rb 2.2 Alternaria dauci – blotter (PDF)

Rb 3.1 Alternaria radicina (PDF)

Cucurbit

Cb 1.1 Acidovorax citrulli – Grow-out (PDF)

Cb 1.5 Acidovorax citrulli – Seedling PCR (PDF)

Cb 1.6 Acidovorax citrulli – ISHI Method (PDF)

Cb 2.1 Didymella bryoniae – PCR (PDF)

Cb 2.2 Didymella bryoniae – blotter (PDF)

Cb 3.1 Cucumber Green Mottle Mosaic Virus (PDF)

Cb 4.1 Melon Necrotic Spot Virus (PDF)

Cb 5.1 Squash Mosaic Virus (PDF)

Cb 6.1 Fusarium oxysporum f sp niveum (PDF)

Celery

Lcb 1.1 Septoria apiicola (PDF)

Lcb 2.1 Phoma apiicola (PDF)

Spinach

Lcb 3.1 Peronospora farinosa f sp spinaciae – wash test (PDF)

Lcb 3.2 Peronospora farinosa f sp spinaciae – wash test (PDF)

Lcb 4.1 Verticillium dahliae – Freeze blotter (PDF)

Lcb 4.2 Verticillium dahliae – NP-10 agar (PDF)

Lcb 5.1 Cucumber Mosaic Virus (PDF)

Lcb 6.1 Stemphylium botryosum fsp spinacia (PDF)

Coriander

Lcb 7.1 Pseudomonas syringae pv coriandricola (PDF)

Lettuce

Lcb 8.1 Lettuce Mosaic Virus – Bioassay on chenopodium (PDF)

Lcb 8.2 Lettuce Mosaic Virus – ISHI Method (PDF)

Corn Salad

Lcb 9.1 Acidovorax valerianellae (PDF)

Tomato/Pepper

So 1.1 Xanthomonas spp. – pepper (PDF)

So 1.2 Xanthomonas spp. – tomato (PDF)

So 2.1 Clavibacter michiganensis ssp michiganensis – tomato (PDF)

So 3.1 Pseudomonas syringae pv tomato – tomato (PDF)

So 4.1 Pepino Mosaic Virus – tomato (PDF)

So 5.1 Tobamovirus – tomato-pepper (PDF)

So 6.1 Pospiviroids – tomato-pepper (PDF)

So 7.1 ToBRFV – tomato-pepper (PDF)

So 8.1 ToMMV – tomato-pepper (PDF)

Onion

On 1.1 Alternaria porri (PDF)

On 2.1 Stemphylium vesicarium (PDF)

Maize

Mz 1.1 Stenocarpella maydis (PDF)

Mz 2.1 Cochliobolus carbonum (PDF)

Mz 3.1 Cochliobolus heterostrophus (PDF)

Mz 4.1 U. maydis and S. reiliana (PDF)

Mz 5.1 Sclerophthora macrospora (PDF)

Mz 6.1 Peronosclerospora sorghi (PDF)

Mz 7.1 Fusarium (PDF)

Mz 8.1 Penicillium oxalicum (PDF)

Mz 9.1 Clavibacter michiganensis ssp nebraskensis (PDF)

Mz 10.1 Pantoea stewartii (PDF)

Mz 11.1 Maize Dwarf Mosaic Virus (PDF)

Mz 12.1 Maize Chlorotic Mottle Virus (PDF)

Mz 13.1 Cercospora zeae-maydis (PDF)

Mz 14.1 Kabatiella zeae (PDF)

Mz 15.1 Phyllosticta maydis (PDF)

Soybean

Sb 1.1 Cercospora kikuchii – Agar plate (PDF)

Sb 1.2 Cercospora kikuchii – Blotter (PDF)

Sb 2.1 Phomopsis-Diaporthe – Agar plate (PDF)

Sb 2.2 Phomopsis-Diaporthe – Blotter (PDF)

Sb 3.1 Sclerotinia sclerotiorum (PDF)

Sb 4.1 Pseudomonas syringae pv glycinea – Soaked bulk seed (PDF)

Sb 4.2 Pseudomonas syringae pv glycinea – Ground bulk seed (PDF)

Sb 5.1 Tobacco Ringspot Virus (PDF)

Sb 6.1 Tomato Ringspot Virus (PDF)

Sb 7.1 Soybean Mosaic Virus (PDF)

Sb 8.1 Bean Pod Mottle Virus (PDF)

Curtobacterium flaccumfaciens pv flaccumfaciens (PDF)

Archived Methods

“Archived methods” are methods that were classified as Temporary Standard methods (B methods) but were not listed on the accreditation of any NSHS participants. These methods have been removed from the official NSHS list of methods and are not to be used for phytosanitary certification unless separate APHIS authorization is given.

Be 1.1 Pseudomonas syringae pv. phaseolicola (syn: P. savastanoi pv phaseolicola)

Be 2.2 Pseudomonas syringae pv syringae

Be 2.3 Pseudomonas syringae pv syringae

Be 4.1 Curtobacterium flaccumfaciens pv flaccumfaciens

Be 4.2 Curtobacterium flaccumfaciens pv flaccumfaciens

Cb 1.2 Acidovorax avenae ssp citrulli – Seminis PCR

Cb 1.3 Acidovorax avenae ssp. citrulli – Syngenta SYBR PCr

Cb 1.4 Acidovorax avenae ssp. citrulli – Monsanto PCR

Cf 7.1 Gibberella zeae – maize

Cf 7.2 Gibberella zeae – maize

Cf 8.1 Fusarium moniliforme – maize

Sb 1.1 Curtobacterium flaccumfaciens pv flaccumfaciens – soybean