National Seed Health System

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ARCHIVED    Sb 1.1 – Curtobacterium flaccumfaciens pv flaccumfaciens – soybean

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PATHOGEN: Curtobacterium flaccumfaciens pv. flaccumfaciens (syn: Corynebacterium flaccumfaciens pv. flaccumfaciens)
HOST: Soybean (Glycine max)
COMMON NAME: bacterial tan spot/wilt
METHOD:  Sb 1.1 Grow-out method (Dunleavy, 1986)
METHOD CLASS: TEMPORARY STANDARD (B)
SAMPLE:  1000 seeds

PROCEDURE:

    1. Surface-sterilized soybean seeds are planted in sterile soil.
    2. They are grown at 30°C under growth-chamber conditions until characteristic large tan-colored lesions leaf symptoms are visible.

REFERENCES:

Dunleavy, J. M. 1986. Effect of temperature on systemic spread of tan spot of soybean from seed to unifoliate leaves. Phytopathology. 76:1079.