Archived 02.21.2025 Be 4.2 – Curtobacterium flaccumfaciens pv. flaccumfaciens
| Version | 1.0 |
|---|---|
| Date | 12/2012 [Archived 02.21.2025] |
| Pathogen | Curtobacterium flaccumfaciens pv. flaccumfaciens (syn: Corynebacterium flaccumfaciens pv flaccumfaciens) |
| Host | common bean (Phaseolus vulgaris) |
| Common Name | bacterial wilt |
| Method | Be 4.2 Selective Media Assay (Maringoni et al., 2006) |
| Method Class | Temporary Standard (B) |
| Sample | 200g seeds |
Procedure:
- Soak 200g samples of bean seeds, in 600 mL sterilized distilled water for 24 h at 5°C.
- Stir the liquid and then use for seeding by streaking with a wire loop onto the surface of the semi-selective culture medium.
- Incubate at 28 – 30°C for 96 to 120 hours and compare the colony characteristics to pure standard isolate (they used Feij-2634)
CFFSM Recipe
| Reagent | Quantity |
|---|---|
| Peptone | 5g |
| Meat Extract | 3g |
| Sucrose | 15g |
| Agar | 5g |
| Skim Milk Powder* | 0.05g |
| Congo Red* | 0.01g |
| Chlorothalonil* | 0.01g |
| Thiophanate Methyl* | 0.01g |
| Nalidixic Acid* | 0.01g |
| Nitrofurantoin* | 0.01g |
| Oxacillin* | 0.001g |
| Sodium Azide* | 0.001g |
| Distilled Water | 1L |
*added after autoclaving the basal medium
References:
Maringoni, A. C. and Camara, R. C. 2006. Curtobacterium flaccumfaciens pv. flaccumfaciens detection in bean seeds using a semi-selective culture medium. Brazilian Journal of Microbiology. 37: 451-455.