Archived Cf 7.2 – Gibberlla zeae
| Version | - |
|---|---|
| Date | - |
| Pathogen | Gibberella zeae |
| Host | Maize (Zea mays) |
| Common Name | Gibberella ear and stalk rot |
| Method | Cf 7.2 Blotter (Singh et al., 1974) |
| Method Class | Temporary Standard (B) |
| Sample | 400 seeds |
Procedure:
- Four replicates of 100 seeds are surface disinfected in 2.0% sodium hypochlorite.
- Incubation on a damp blotter at 20°C for 24 h, followed.
- Freezing at -20°C for 24 h.
- Incubation for 7 days at 26°C under cycles of 12 h NUV light/12 h darkness.
References:
Singh, D. V., Mathur, S. B. and Neergaard, P. 1974. Seed health testing of maize. Evaluation of testing techniques with particular reference to Drechslera maydis. Seed Sci. Technol. 2:349-365.