Br 2.1 Phoma lingam2019-04-25T14:50:27-05:00

National Seed Health System

Br 2.1 Phoma lingam

DATE: 1/1/2019
PATHOGEN: Phoma lingam [sexual stage: Leptosphaeria maculans and Plenodomus (Leptosphaeria) biglobosa]
HOST: Brassica sp.
COMMON NAME: black leg; canker; dry rot
METHOD: Br 2.1  Freeze Blotter Section of (ISTA 7-004: ver 2.0) Detection of Leptosophaeria maculans and Plenodomus biglobosus on Brassica seed) (2019)
SAMPLE: 1000 seeds minimum

Version 1.1: Concentration of Botran solution corrected from 0.5% to 0.05%; made from the 75% WP.

Version 1.2: ISTA 7-004 describes both a freeze-blotter method and a malt agar plating method. Only the freeze-blotter method is approved by NSHS at this time. The use of Botran solution for Rhizopus control is allowed.


International Rules for Seed Testing. Annexe to Chapter 7: Seed Health Testing Methods. 2019 7-004: ver 2.0 Detection of Leptosphaeria maculans and Plenodomus biglobosus on Brassica spp. seed.