National Seed Health System
Sb 3.1 Sclerotinia sclerotiorum
|PATHOGEN: Sclerotinia sclerotiorum|
|HOST: Soybean (Glycine max)|
|COMMON NAME: Sclerotinia stem rot|
|METHOD: Sb 3.1 Culture plate (Totir, 2000) (formerly Sf 3.1)|
|METHOD CLASS: STANDARD (A)|
|SAMPLE: 400 seeds|
1. Four sub-samples of 100 seeds are surface sterilized in 1.75% NaOCl for 30 seconds.
2. Seeds are rinsed three times in sterile water.
3. Incubate seeds on potato dextrose agar for 10 days at 25ºC (5 seeds/plate).
4. Seeds with characteristic white mycelium of Sclerotinia sclerotiorum are marked after 3, 5 and 7 days to account for overgrowth of colonies compromising the final count.
5. A final count is made of seeds with characteristic white mycelium and/or large black sclerotia, at 10 days.
Totir, C. 2000. Seed transmission and control of Sclerotinia sclerotiorum in soybean seeds. Ms. Thesis, Iowa State University, Ames, IA.