Sb 2.2 Phomopsis-Diaporthe – Blotter 2017-05-19T16:32:45+00:00

National Seed Health System

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Sb 2.2  Phomopsis-Diaporthe – Blotter

VERSION: 1.0
DATE: 2001
PATHOGEN: Phomopsis/Diaporthe complex
HOST: Soybean (Glycine max)
COMMON NAME: pod and stem blight; seed decay; stem canker
METHOD: Sb 2.2 Blotter (McGee and Nyvall, 1984) (formerly Sf 2.2)
METHOD CLASS: STANDARD (A)
SAMPLE: 400 seeds

PROCEDURE:

1. Two sterilized blotters are placed in plastic boxes.

2. Moisten blotters with 80ml of sterile water, containing 40mg of 2,6 dichloro-6-nitroaniline (Botran 75W).

3. Four sets of 100 seeds are surface sterilized in 1% NaOCl for 30 seconds and then rinsed in sterile water.

4. Seeds are incubated at 25°C for 10 days under continuous light.

5. Seeds are evaluated for the presence of fungi in the Phomopsis/Diaporthe complex characteristic dense, white fungal growth.

REFERENCES:

McGee, D. C., Brandt, C. L. and Burris, J. S. 1980. Seed mycoflora of soybeans relative to fungal interactions, seedling emergence, and carry-over of pathogens to subsequent crops. Phytopathology. 70(7):615-617.

McGee, D. C. and Nyvall, R. F. 1984. Soybean seed health. Coop. Ext. Serv. Iowa State Univ. Pm- 990.