National Seed Health System
Sb 11.1 Phytophthora megasperma fp. glycinea
|PATHOGEN: Phytophthora megasperma fp. glycinea (syn: Phytophthora sojae)|
|HOST: Soybean (Glycine max)|
|COMMON NAME: Phytophthora root and stem rot|
|METHOD: Sb 11.1 Culture Plate (formerly Sf 6.1)|
|METHOD CLASS: REGULATED NON-SEEDBORNE PEST|
|SAMPLE: : 400 seeds|
1. Randomly select 400 seeds; 4 replicates of 100.
2. Wash thoroughly in running water to remove chemical seed treatment.
3. Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 3 minutes. Triple rinse with sterile water.
4. Aseptically place seeds on PDA (potato dextrose agar), usually 5 to 10 seeds per petri plate.
5. Inoculate one PDA plate per replicate with a known culture of P. megasperma fp. glycinea.
6. Incubate samples at 25°C with 12 hours of light per day for 7 days.
7. Examine the plates for colonies typical of P. megasperma fp. glycinea.
8. Suspect mycelium and/or fruiting bodies should be examined under magnification to confirm the identity.
McGee, D. C. 1991. Soybean Diseases. APS Press, St. Paul, MN.
CABI. Crop Protection Compendium cabi.org/cpc/datasheet/40974