National Seed Health System
Sb 10.1 Phialophora gregatum
|PATHOGEN: Phialophora gregatum (syn: Cephalosporium gregatum; Phialophora gregata; Cadophora gregata)|
|HOST: Soybean (Glycine max)|
|COMMON NAME: brown stem rot|
|METHOD: Sb 10.1 Culture Plate (formerly Sf 5.1)|
|METHOD CLASS: REGULATED NON-SEEDBORNE PEST|
|SAMPLE: : 400 seeds|
1. Randomly select 400 seeds; 4 replicates of 100.
2. Wash thoroughly in running water to remove chemical seed treatment.
3. Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 3 minutes. Triple rinse with sterile water.
4. Aseptically place seeds on PDA (potato dextrose agar), usually 5 to 10 seeds per petri plate.
5. Inoculate one PDA plate per replicate with a known culture of P. gregatum.
6. Incubate samples at 25°C with 12 hours of light per day for 7 days.
7. Examine the plates for colonies typical of P. gregatum.
8. Suspect mycelium and/or fruiting bodies should be examined under magnification to confirm the identity.
McGee, D. C. 1991. Soybean Diseases. APS Press, St. Paul, MN.