Sb 10.1 Phialophora gregatum 2017-05-19T17:32:02+00:00

National Seed Health System

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Sb 10.1   Phialophora gregatum

VERSION: 1.0
DATE: 2001
PATHOGEN: Phialophora gregatum (syn: Cephalosporium gregatum; Phialophora gregata; Cadophora gregata)
HOST: Soybean (Glycine max)
COMMON NAME: brown stem rot
METHOD: Sb 10.1 Culture Plate (formerly Sf 5.1)
METHOD CLASS: REGULATED NON-SEEDBORNE PEST
SAMPLE: : 400 seeds

PROCEDURE:

1. Randomly select 400 seeds; 4 replicates of 100.

2. Wash thoroughly in running water to remove chemical seed treatment.

3. Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 3 minutes. Triple rinse with sterile water.

4. Aseptically place seeds on PDA (potato dextrose agar), usually 5 to 10 seeds per petri plate.

5. Inoculate one PDA plate per replicate with a known culture of P. gregatum.

6. Incubate samples at 25°C with 12 hours of light per day for 7 days.

7. Examine the plates for colonies typical of P. gregatum.

8. Suspect mycelium and/or fruiting bodies should be examined under magnification to confirm the identity.

REFERENCES:

McGee, D. C. 1991. Soybean Diseases. APS Press, St. Paul, MN.