Sb 1.2 Cercospora kikuchii – Blotter 2017-05-19T16:23:17+00:00

National Seed Health System

 Sb 1.2  Cercospora kikuchii – Blotter

VERSION: 1.0
DATE: 2001
PATHOGEN: Cercospora kikuchii
HOST: Soybean (Glycine max)
COMMON NAME: purple seed stain
METHOD: Sb 1.2 Culture Plate (McGee and Nyvall, 1984) (formerly Sf 1.2)
METHOD CLASS: STANDARD (A)
SAMPLE: 400 seeds

PROCEDURE:

1. Seeds are surface sterilized in 1% sodium hypochlorite (NaOCl) for 30 seconds and then rinsed in sterile water.

2. Incubate on a moistened blotter at 25°C for 10 days under continuous light.

3. Seeds are evaluated for the presence of Cercospora kikuchii as indicated by purple staining on the seed coat under fungal growth.

REFERENCES:

McGee, D. C., Brandt, C. L. and Burris, J.S. 1980. Seed mycoflora of soybeans relative to fungal interactions, seedling emergence, and carry-over of pathogens to subsequent crops. Phytopathology. 70(7):615-617.

McGee, D. C. and Nyvall, R. F. 1984. Soybean seed health. Coop. Ext. Serv. Iowa State University. Pm-990.