National Seed Health System
Sb 1.2 Cercospora kikuchii – Blotter
|PATHOGEN: Cercospora kikuchii|
|HOST: Soybean (Glycine max)|
|COMMON NAME: purple seed stain|
|METHOD: Sb 1.2 Culture Plate (McGee and Nyvall, 1984) (formerly Sf 1.2)|
|METHOD CLASS: STANDARD (A)|
|SAMPLE: 400 seeds|
1. Seeds are surface sterilized in 1% sodium hypochlorite (NaOCl) for 30 seconds and then rinsed in sterile water.
2. Incubate on a moistened blotter at 25°C for 10 days under continuous light.
3. Seeds are evaluated for the presence of Cercospora kikuchii as indicated by purple staining on the seed coat under fungal growth.
McGee, D. C., Brandt, C. L. and Burris, J.S. 1980. Seed mycoflora of soybeans relative to fungal interactions, seedling emergence, and carry-over of pathogens to subsequent crops. Phytopathology. 70(7):615-617.
McGee, D. C. and Nyvall, R. F. 1984. Soybean seed health. Coop. Ext. Serv. Iowa State University. Pm-990.