National Seed Health System
Pe 1.2 Ascochyta pinodes – agar plate
|PATHOGEN: Ascochyta pinodes (sexual: Mycosphaerella pinodes)|
|HOST: pea (Pisum sativum)|
|COMMON NAME: blight|
|METHOD: Pe 1.2 Semi-selective agar (Chen et al., 2010)|
|METHOD CLASS: TEMPORARY STANDARD (B)|
|SAMPLE: 50 seeds/seed lot|
1. Seeds are aseptically placed onto SC semi-selective medium, five seeds per dish.
2. After incubation at 24°C for five days, the number of seeds infected by M. pinodes in each seedlot is recorded.
30 g soluble starch,
3 g casein,
25 µg/ml propamocarb,
100 µg/mlstreptomycin sulfate and
200 µg/mlneomycin sulfate per liter of water.
Mei-Hsing Chen, Wen-Chuan Chung, Hung-Chang Huang and Jenn-Wen Huang. 2010. Development of a semiselective medium for detection of Mycosphaerella pinodes in soil, plant debris and seed. Canadian Journal of Plant Pathology 32(3):342- 350.