On 2.1 Stemphylium vesicarium 2017-05-20T17:09:38+00:00

National Seed Health System

On 2.1     Stemphylium vesicarium

VERSION: 1.0
DATE: 12/2013
PATHOGEN: Stemphylium vesicarium
HOST: onion (Allium cepa)
COMMON NAME: leaf blight
METHOD: On 2.1 Stemphylium vesicarium
METHOD CLASS: TEMPORARY STANDARD (B)
SAMPLE: : 200 seeds

PROCEDURE:

1. Surface sterilize seed with 1% NaOCl.

2. Place seeds onto Potato Dextrose agar.

3. Incubate at 20oC at 12 hour dark/12 hour UV light for 6 days.

4. Inspect for presence of sporulation.

REFERENCES:

Aveling, T. A. S., Snyman, H. G. and Naude, S. P. 1993. Evaluation of seed treatments for reducing Alternaria porri and Stemphylium vesicarium on onion seed. Plant Disease. 77(10): 1009-1011.