National Seed Health System
Mz 4.1 U. maydis and S. reiliana
|PATHOGEN: Ustilago maydis/Sphacelotheca reiliana (syns: Ustilago zeae/Sporisorium holci-sorghi)|
|HOST: Maize (Zea mays)|
|COMMON NAME: common/head smut|
|METHOD: Mz 4.1 Washing test (McGee, 1988) (formerly Cf 4.1)|
|METHOD CLASS: Standard (A)|
|SAMPLE: : 400 seeds|
1. Wash 400 seeds in sterile water for 15 minutes in a shaker.
2. Centrifuge at 3000 rpm for 15 minutes.
3. Remove the pellet and examine with a light microscope to confirm the identity of teliospores as described by Ainsworth (1965a) for U. maydis and Ainsworth (1965b) for S. reiliana
Ainsworth, G. C. (1965a) Ustilago maydis. CMI Descriptions of Pathogenic Fungi and Bacteria. No. 79. Wallingford, UK: CAB International.
Ainsworth, G. C. (1965b) Sphacelotheca reiliana. CMI Descriptions of Pathogenic Fungi and Bacteria. No. 73. International Mycological Institute: Kew, UK.
McGee, D.C., 1988. Maize diseases. A reference source for seed technologists. St. Paul, Minnesota, USA. APS Press. 149 pp.