Mz 15.1 Phyllosticta maydis 2017-05-20T13:01:28+00:00

National Seed Health System

Mz 15.1   Phyllosticta maydis

VERSION: 1.0
DATE: 2001
PATHOGEN: Phyllosticta maydis (sexual: Mycosphaerella zeae-maydis)
HOST: Maize (Zea mays)
COMMON NAME: yellow leaf blight
METHOD: Mz 15.1 Culture Plate (formerly Cf 12.1)
METHOD CLASS: REGULATED NON-SEEDBORNE PEST
SAMPLE: 400 seeds

PROCEDURE:

1. Randomly select 400 seeds; 4 replicates of 100.

2. Wash thoroughly in running water to remove chemical seed treatment.

3. Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 3 minutes. Triple rinse with sterile water.

4. Aseptically place seeds on PDA (potato dextrose agar), usually 5 to 10 seeds per petri plate.

5. Inoculate one PDA plate per replicate with a known culture of P. maydis.

6. Incubate samples at 25°C with 12 hours of light per day for 7 days.

7. Examine the plates for colonies typical of P. maydis.

8. Suspect mycelium and/or fruiting bodies should be examined under magnification to confirm the identity.

REFERENCES:
McGee, D. C. 1988. Maize Diseases. APS Press, St. Paul, MN.