Mz 14.1 Kabatiella zeae 2017-05-20T13:34:04+00:00

National Seed Health System

Mz 14.1   Kabatiella zeae

VERSION: 1.0
DATE: 2001
PATHOGEN: Kabatiella zeae (syn: Aureobasidium zeae)
HOST: Maize (Zea mays)
COMMON NAME: eyespot
METHOD: Mz 14.1 Culture Plate (formerly Cf 11.1)
METHOD CLASS: REGULATED NON-SEEDBORNE PEST
SAMPLE: : 400 seeds

PROCEDURE:

1. Randomly select 400 seeds; 4 replicates of 100.

2. Wash thoroughly in running water to remove chemical seed treatment.

3. Immerse seeds in 1.0% (v/v) solution of sodium hypochlorite for 3 minutes. Triple rinse with sterile water.

4. Aseptically place seeds on 4% malt agar, usually 5 to 10 seeds per petri plate.

5. Inoculate one plate per replicate with a known culture of K. zeae.

6. Incubate samples at 25°C with 12 hours of light per day for 7 days.

7. Examine the plates for colonies typical of K. zeae.

8. Suspect mycelium and/or fruiting bodies should be examined under magnification to confirm the identity.

REFERENCES:

McGee, D. C. 1988. Maize Diseases. APS Press, St. Paul, MN

CABI. Crop Protection Compendium cabi.org/cpc/datasheet/29297