Lcb 8.1 Lettuce Mosaic Virus – Bioassay on chenopodium 2017-05-20T17:17:01+00:00

National Seed Health System

Lcb 8.1   Lettuce Mosaic Virus – Bioassay on chenopodium

VERSION: 1.0
DATE: 2001
PATHOGEN: Lettuce Mosaic Virus (LMV)
HOST: lettuce (Lactuca sativa)
COMMON NAME: 
METHOD: Lcb 8.1 Chenopodium Assay (Kimble et al., 1975) (formerly Cv 1.1)
METHOD CLASS: STANDARD (A)
SAMPLE: : 30,000 seeds

PROCEDURE:

1. Grind sub-samples of 500 lettuce seeds (total sample 30,000 seeds) in 4ml 0.05M phosphate buffer pH 7.7, containing 0.1% thioglycolic acid.

2. Mechanically inoculate five Chenopodium quinoa seedlings with each extract (within 16 minutes of making the extract).

3. Observe indicator plants for symptoms 18-20 days after inoculation (chlorotic local lesions followed by systemic yellow vein-netting and leaf distortion).

REFERENCES:

Kimble, K. A., Grogan, R. G., Greathead, A. S., Paulus, A. O. and House, J. K. 1975. Development, application, and comparison of methods for indexing lettuce seed for mosaic virus in California. Plant Disease Reporter. 59(6):461-464.