Lcb 8.1 Lettuce Mosaic Virus – Bioassay on chenopodium
|PATHOGEN: Lettuce Mosaic Virus (LMV)|
|HOST: lettuce (Lactuca sativa)|
|METHOD: Lcb 8.1 Chenopodium Assay (Kimble et al., 1975) (formerly Cv 1.1)|
|METHOD CLASS: STANDARD (A)|
|SAMPLE: : 30,000 seeds|
1. Grind sub-samples of 500 lettuce seeds (total sample 30,000 seeds) in 4ml 0.05M phosphate buffer pH 7.7, containing 0.1% thioglycolic acid.
2. Mechanically inoculate five Chenopodium quinoa seedlings with each extract (within 16 minutes of making the extract).
3. Observe indicator plants for symptoms 18-20 days after inoculation (chlorotic local lesions followed by systemic yellow vein-netting and leaf distortion).
Kimble, K. A., Grogan, R. G., Greathead, A. S., Paulus, A. O. and House, J. K. 1975. Development, application, and comparison of methods for indexing lettuce seed for mosaic virus in California. Plant Disease Reporter. 59(6):461-464.