Cf 7.2 Gibberella zeae – ARCHIVED 2018-08-10T14:44:07+00:00

National Seed Health System

ARCHIVED    Cf  7.2 – Gibberlla zeae

VERSION: 
DATE: 
PATHOGEN: Gibberella zeae
HOST: Maize (Zea mays)
COMMON NAME: Gibberella ear and stalk rot
METHOD: Cf 7.2 Blotter (Singh et al., 1974)
METHOD CLASS: TEMPORARY STANDARD (B)
SAMPLE:  400 seeds

PROCEDURE:

    1. Four replicates of 100 seeds are surface disinfected in 2.0% sodium hypochlorite.
    2. Incubation on a damp blotter at 20°C for 24 h, followed.
    3. Freezing at -20°C for 24 h.
    4. Incubation for 7 days at 26°C under cycles of 12 h NUV light/12 h darkness.

REFERENCES:

Singh, D. V., Mathur, S. B. and Neergaard, P. 1974. Seed health testing of maize. Evaluation of testing techniques with particular reference to Drechslera maydis. Seed Sci. Technol. 2:349-365.