Br 2.1 Phoma lingam
|PATHOGEN: Phoma lingam [sexual stage: Leptosphaeria maculans and Plenodomus (Leptosphaeria) biglobosa]|
|HOST: Brassica sp.|
|COMMON NAME: black leg; canker; dry rot|
|METHOD: Br 2.1 Freeze Blotter Section of (ISTA 7-004: Detection of Leptosophaeria maculans and Plenodomus biglobosus on Brassica seed)|
|METHOD CLASS: STANDARD (A)|
|SAMPLE: 1000 seeds minimum|
Version 1.1: Concentration of Botran solution corrected from 0.5% to 0.05%; made from the 75% WP.
Version 1.2: ISTA 7-004 describes both a freeze-blotter method and a malt agar plating method. Only the freeze-blotter method is approved by NSHS at this time. The use of Botran solution for Rhizopus control is allowed.
International Rules for Seed Testing. Annexe to Chapter 7: Seed Health Testing Methods. 2018 7-004: ver 2.0 Detection of Leptosphaeria maculans and Plenodomus biglobosus on Brassica spp. seed.