Br 2.1 Phoma lingam 2018-04-03T15:24:30+00:00

National Seed Health System

Br 2.1 Phoma lingam

VERSION: 1.2
DATE: 2018
PATHOGEN: Phoma lingam [sexual stage: Leptosphaeria maculans and Plenodomus (Leptosphaeria) biglobosa]
HOST: Brassica sp.
COMMON NAME: black leg; canker; dry rot
METHOD: Br 2.1  Freeze Blotter Section of (ISTA 7-004: Detection of Leptosophaeria maculans and Plenodomus biglobosus on Brassica seed)
METHOD CLASS: STANDARD (A)
SAMPLE: 1000 seeds minimum

REVISION HISTORY:
Version 1.1: Concentration of Botran solution corrected from 0.5% to 0.05%; made from the 75% WP.

Version 1.2: ISTA 7-004 describes both a freeze-blotter method and a malt agar plating method. Only the freeze-blotter method is approved by NSHS at this time. The use of Botran solution for Rhizopus control is allowed.

REFERENCES:

International Rules for Seed Testing. Annexe to Chapter 7: Seed Health Testing Methods. 2018 7-004: ver 2.0 Detection of Leptosphaeria maculans and Plenodomus biglobosus on Brassica spp. seed.