Be 4.2 Curtobacterium flaccumfaciens pv. flaccumfaciens 2017-06-14T17:00:05+00:00

National Seed Health System

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Be 4.2 Curtobacterium flaccumfaciens pv. flaccumfaciens

VERSION: 1.0
DATE: 12/2012
PATHOGEN: Curtobacterium flaccumfaciens pv. flaccumfaciens (syn: Corynebacterium flaccumfaciens pv flaccumfaciens)
HOST: common bean (Phaseolus vulgaris)
COMMON NAME: bacterial wilt
METHOD: Be 4.2 Selective Media Assay (Maringoni et al., 2006)
METHOD CLASS: TEMPORARY STANDARD (B)
SAMPLE: 200g seeds

PROCEDURE:

1. Soak 200g samples of bean seeds, in 600 mL sterilized distilled water for 24 h at 5°C.

2. Stir the liquid and then use for seeding by streaking with a wire loop onto the surface of the semi-selective culture medium.

3. Incubate at 28 – 30°C for 96 to 120 hours and compare the colony characteristics to pure standard isolate (they used Feij-2634).

CFFSM Recipe:
Peptone 5g,
meat extract 3g,
sucrose 5g,
agar 15g,
skim milk powder* 5g,
Congo red* 0.05g,
chlorothalonil* 0.01g,
thiophanate methyl* 0.01g,
nalidixic acid* 0.01g,
nitrofurantoin* 0.01g,
oxacillin* 0.001g,
sodium azide* 0.001g,
and distilled water 1L. *added after autoclaving the basal medium

REFERENCES:

Maringoni, A. C. and Camara, R. C. 2006. Curtobacterium flaccumfaciens pv. flaccumfaciens detection in bean seeds using a semi-selective culture medium. Brazilian Journal of Microbiology. 37: 451-455.